New School Year and lots of IDEAS
This summer, I was given the opportunity to do research in Dr. Joshua Rabinowitz laboratory at Princeton University. It was a great experience. I was working with a different area of Science (Biochemistry) and a different model organism (E. coli). I learned many new techniques and it presented me to a new way of analyzing the data, which helps me expand my knowledge as a scientist. It was very challenging at the beginning but with effort and dedication I finished the summer more than satisfied with my work.
During this semester, at Dr. Carlos I. Gonzalez laboratory, I expect to further characterize the role of Mex67 and Upf1 in translation. In order to accomplish this, I plan to make growth curves in different conditions and at different temperatures to view growth defects. Also, I expect to perform many polysome profiles followed by Western Blots at different temperatures in order to confirm my previous data and to have an idea of the molecular behavior of my strains.
As you may notice, my project is directed, since last semester, toward the elucidation of the role of Mex67 and Upf1 in translation, which is my main topic. As a new technique, I plan to make some growth curves in order to measure quantitatively the growth defects that we have noticed before.
